Procomcure offers comprehensive microbiome analysis services using next-generation sequencing (NGS) to provide a complete and detailed view of the composition, diversity, and function of microbial communities. Our services are tailored to various areas of research, including human, animal and environmental health, as well as the food and agricultural industry.

Specific Services :

We offer a complete workflow for microbiome analysis, including:

• DNA extraction: This is implied, but we also offer it as a standalone service.
• Preparing Libraries: Adaptation of DNA for NGS sequencing.
• NGS Sequencing: Massive parallel sequencing to obtain microbial DNA readings.
• Bioinformatic analysis:
  • Identification and Quantification of Taxa: We determine the taxonomic composition of the microbiome, identifying and quantifying the different bacteria, archaea, fungi and viruses present.
  • Alpha and Beta Diversity Analysis: We assess microbial diversity within a sample (alpha) and between different samples (beta), providing information on the richness and uniformity of communities.
  • Functional Analysis: Prediction of metabolic functions and pathways present in the microbiome, allowing us to understand their impact on the host or environment.
  • Phylogenetic analysis: Reconstruction of the evolutionary relationships between the different microbial species.
  • Biomarker Analysis: Identification of microorganisms or genes associated with specific conditions.

Our microbiome analysis services are applicable to a wide range of studies:

• Human Health: Research on the relationship between the microbiome and various diseases, such as inflammatory bowel diseases, obesity, diabetes, cardiovascular disease, and neurological disorders.
• Animal Health: Studies on the animal microbiome and its influence on health, productivity and disease resistance.
• Environmental Health: Analysis of the microbiome in different ecosystems, such as soil, water, and air, to understand its role in biogeochemical cycles and bioremediation.
• Food and Agricultural Industry: Characterization of the microbiome in food and crops to improve food security, product quality, and agricultural productivity.

• Gut Microbiome Test: The analysis of the gut microbiome allows individuals to gain insight into their own gut microbiota. This test can provide information about imbalances in the gut microbiota (dysbiosis) and their possible relationship with digestive health and general well-being.
• Hair Microbiome Test:Your scalp is home to a complex and dynamic ecosystem of billions of microorganisms known as the hair microbiome. This microbiome plays a crucial role in the health and appearance of your hair. A proper balance of bacteria on the scalp is critical for shiny, strong hair and a healthy scalp.
• Female Microbiome Test:The vagina is a complex organ that houses a dynamic ecosystem of microorganisms, primarily bacteria, known as the vaginal microbiome. This microbiome plays a fundamental role in women’s intimate health, influencing such important aspects as infection prevention, fertility and general well-being.
• Oral Microbiome Test: The oral microbiome, the complex community of microorganisms that inhabit our mouth, plays a crucial role in our health, both oral and general. Our lifestyle habits directly influence this delicate ecosystem.
• Skin Microbiome Test:Your skin is home to a complex ecosystem of billions of microorganisms, known as the microbioskin. This microbiome plays a critical role in the health and appearance of your skin.

Procomcure offers a wide range of high-quality consumables and reagents designed to optimize molecular biology workflows and ensure the quality and reproducibility of experimental results. Its catalog includes:

• PCR Consumables: Procomcure offers a variety of PCR consumables to suit different laboratory needs and formats:
  • PCR Plates: Available in 96- and 384-well formats, with different edge options (bordered or borderless) and color. Made from high-quality polypropylene for efficient heat transfer and low DNA/RNA binding.
  • Deep Well Plates: Ideal for the storage and handling of large sample volumes. Available in different capacities and formats.
  • PCR Tubes: Individual tubes and tube strips with individual or strip caps. Available in different volumes and with different lid options (flat or domed).
  • Plate Seals: Adhesive seals and heat-sealable seals to prevent evaporation and cross-contamination during PCR.

These consumables are compatible with Procomcure PCR reagents and are used in a variety of applications, including preparing libraries for NGS sequencing and performing PCR for pathogen detection or genotyping.

• DNA/RNA Isolation Kits: Procomcure offers kits for efficient and rapid DNA and RNA extraction from a wide variety of sample types, including:
  • Genomic DNA Kits: For the extraction of high-quality DNA from blood, tissue, cells, saliva, and other types of samples.
  • Total RNA Kits: For the extraction of total RNA, including messenger RNA (mRNA), ribosomal RNA (rRNA), and small RNAs.
  • Viral DNA/RNA Kits: For the extraction of viral nucleic acids from different types of samples.
  • FFPE Kits: Kits optimized for DNA and RNA extraction from formalin-fixed, paraffin-embedded samples.

The high quality of the DNA and RNA extracted with these kits is crucial for the success of downstream applications such as NGS sequencing (WGS, WES, RNA-Seq, targeted sequencing, and metagenomics) and PCR. Procomcure offers optimized kits for different types of samples, ensuring the obtaining of high purity and high yield nucleic acids, which is essential for reliable results in sequencing and bioinformatic analysis services.

• PCR Reagents: Procomcure offers a complete line of PCR reagents, including:
  • DNA polymerases: High-fidelity polymerases, hot-start polymerases, and standard PCR polymerases. Optimized for different applications, such as high-fidelity PCR, multiplex PCR and RT-PCR.
  • Master Mixes: Ready-to-use mixtures that contain all the components needed for PCR, including polymerase, dNTPs, buffer, and magnesium ions. Available in different formulations to suit different applications.
  • dNTPs: High quality deoxynucleotide triphosphate.
  • PCR buffers: Buffers optimized for different PCR applications.
  • PCR Additives: Additives that improve PCR performance under challenging conditions, such as the presence of inhibitors.

These reagents are critical for a variety of applications, including:

• Preparing libraries for NGS sequencing: Procomcure’s high-fidelity polymerases are ideal for amplifying libraries for sequencing, ensuring high fidelity and a low error rate.
• Validation of sequencing results: PCR is often used to validate genetic variants identified by NGS sequencing.
• Pathogen detection: Procomcure PCR kits and reagents can be used for rapid and sensitive detection of pathogens, complementing metagenomics services.
• Gene expression analysis by RT-PCR: RT-PCR is used to quantify gene expression, complementing the data obtained by RNA-Seq.

Procomcure’s ability to overcome PCR inhibitors, as mentioned in one of the search results (“PCR problem-solver kit for PCR test kit manufacturers and molecular diagnostics (MDx), highly resistant to a broad field of PCR inhibitors such as heparin (blood samples), serum or humic acid (soil / plant samples)”)^,1 is an important added value that benefits customers working with complex samples.

• Sample Collection Kits: Procomcure offers kits for the safe and convenient collection and transport of biological samples:
  • Swab Kits: For the collection of oral, nasal or pharyngeal mucosa samples.
  • Saliva kits: For non-invasive collection of saliva samples.
  • Sample Transport Kits: For the safe transport of samples at room temperature or refrigerated.

Proper sample collection and transport is crucial to the quality of results in all molecular biology applications, including NGS sequencing, microbiome analysis, and PCR. Procomcure kits ensure sample integrity and minimize the risk of contamination.

Emphasis on quality and optimization (with connection to other services):

All Procomcure consumables and reagents are manufactured under strict quality controls to ensure reproducibility and reliability of results. In addition, they offer technical support to help customers select the most suitable products for their specific needs and optimize their experimental protocols, whether preparing samples for sequencing, performing validation PCRs, or performing microbiome analysis.

In addition to reagents and consumables, Procomcure offers a range of analysis equipment that complements its sequencing and PCR services, allowing laboratories to perform diagnostic tests and analyses efficiently and accurately. Here are some examples, reinforcing the connection with other services:

• Molecular Diagnostic Equipment (examples): Procomcure also offers equipment for a variety of other molecular diagnostic applications, including:
  • Conventional thermal cyclers: For standard PCR performance, which can be used in combination with Procomcure PCR reagents for various applications, such as validating sequencing results or detecting pathogens.
  • Capillary electrophoresis systems: For the analysis of DNA and RNA fragments, which can be used to confirm the results obtained by PCR or sequencing.
  • Microplate readers: For ELISA plate reading and other immunological tests, which can complement genetic analysis by offering a more complete view of the patient’s condition.
  • Equipment for the Lactose Test (and other food intolerances): Procomcure provides complete solutions for the detection of lactose intolerance and other food intolerances through genetic analysis. This includes:Genotyping Kits: Specific kits for the detection of genetic variants associated with lactose intolerance (such as the LCT-13910 C >T variant). These kits are based on technologies such as real-time PCR or allelic discrimination PCR and are complemented with Procomcure’s PCR reagents, ensuring the optimization of the reaction.

This approach allows for an accurate genetic diagnosis of lactose intolerance, differentiating between primary adult hypolactasia (the most common form of lactose intolerance) and other causes of lactose malabsorption.

This service is complemented by Procomcure’s technical advice for the interpretation of the results and the design of research studies.

• Real-Time PCR Equipment: Real-time PCR thermal cyclers that enable real-time DNA amplification and quantification, facilitating fast and accurate detection of genetic variants. These kits are essential for lactose testing and other genetic testing, and integrate seamlessly with Procomcure DNA extraction kits for a complete sample-to-result workflow.
• Analysis Software: Specialized software for real-time PCR data analysis, enabling interpretation of results and reporting. This software can even be integrated with Procomcure bioinformatics platforms for deeper analysis and custom reporting.

The equipment offering can include solutions for:

• Pathogen detection: Equipment and kits for the detection of bacteria, viruses and other microorganisms, which are complemented by Procomcure’s metagenomics services for the identification and characterization of complex microbial communities.
• Diagnosis of genetic diseases: Equipment and kits for the detection of mutations associated with genetic diseases, which are integrated with sequencing services (WES, targeted sequencing) for more complete analysis and identification of new variants.
• Molecular oncology: Equipment and kits for the analysis of tumour markers and the detection of somatic mutations in cancer, which are complemented by whole exome sequencing and targeted sequencing services for the identification of relevant variants in oncology.

• Integration with Procomcure services: The analysis equipment offered by Procomcure is designed to integrate with your other services, creating a seamless workflow from sample preparation to data analysis.
  • Extraction – PCR – Analysis: For example, a lab could use a Procomcure DNA/RNA extraction kit, perform PCR with Procomcure reagents in a thermal cycler provided by Procomcure, and then analyze the results with Procomcure analysis software. This integrated workflow minimizes errors, saves time, and ensures compatibility between different components.
  • Sequencing – Validation: The results obtained by NGS sequencing can be validated by PCR using Procomcure equipment and reagents.
  • Metagenomics – Functional Analysis: The data obtained through metagenomics can be complemented with functional analyses using specific equipment and kits.